Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) is one of the most important spectrometric techniques. The NexION® 5000 multi-quadrupole ICP-MS – the first in its category to boast four quads – is innovatively designed to meet and exceed the demanding requirements of ultra-trace elemental applications. It takes ICP-MS performance beyond traditional triple quad to deliver exceptionally low background equivalent concentrations (<1 ppt, even in hot plasma) and outstanding detection limits, key to ensuring accurate, repeatable results. Plus, the NexION 5000 provides superior interference removal, phenomenal stability and unmatched matrix tolerance. NexION 5000 is a powerful method used to conduct quantitative and qualitative analysis.

Features of NexION 5000

ICP-MS detects elements instead of molecules. With the exception of a few elements (such as C, H, N, O, and the noble gasses), all elements can be detected. A specific element serves as a tag for the molecule of interest, thus enabling quantitation of the drug molecule in a complex matrix. The technique is highly linear and can be used quantitatively for a broad concentration range. Sample processing is relatively straightforward with high throughput resulting in fast turnaround times.
Furthermore ICP-MS is frequently used for limit tests of elements, trace elemental analysis, and formulation analysis.
The NexION 5000 is capable of performing multi-element analyzes. Using the ICP-MS, it is possible to measure multiple elements simultaneously in a single analysis.

Ideal Uses of NexION 5000

• Quantitative analysis of minor and trace elements
• Purity certification, cleaning validation, and contamination identification for a broad range of materials and processes of industrial significance. For example, ultra-pure water, cooling water, acids, organic solvents
• Supply chain efficiency support, ranging from raw materials/feedstock qualification, product safety, research and development, production control to failure analysis.

Our NexION 5000 is coupled with Single Cell application module capable of:

• Delivering intact individual cells to the plasma
• An increased transport of larger micron sized objects into the plasma
• Mass of metal per cell (attograms / cell)
• Mass distribution per cell population
• Concentration of cells containing metal or nanoparticles
• Number of nanoparticles per cell
   

• Technical Specifications:

  Quadrupole Ion Deflector (Q0), First Transmission Analyzer Quadrupole (Q1), Quadrupole Universal Cell (Q2), Second Transmission Analyzer Quadrupole (Q3), Mass range: 1-285 amu, 2.5 MHz.Gamma di massa: 1-285 amu, 2,5 MHz
  Resolution: Typical operating resolution 0.7 amu, custom resolution to <0.3 amu
  Quadrupole peak hop (slew) speed: 1.6 M amu/sec
  Quadrupole scan speed: 5000 amu/sec

The Thermo Scientific LTQ XL™ Linear Ion Trap Mass Spectrometer provides accurate mass measurements (15 to 200 m/z; 50 – 2000 m/z; 200 – 4000 m/z) with improved resolution and high sensitivity (low fetomole to high attomole level). The instrument can accumulate, isolate and fragment ions by detecting them through its Secondary Electron Multiplier Detectors (SEM). The instrument can perform different types of scans: Full scan (single mass scan (n = 1) which provides a complete mass spectrum); Selected Ion Monitoring (SIM) (the ions produced in the linear ion trap are accumulated and only the ions with a certain value of m / z are expelled from the ion trap); Selected Reaction Monitoring (SRM) (in the first phase a precursor ion with a certain m / z ratio is accumulated into the trap and further fragmented producing fragment ions, while all the other ions are removed. In the second phase of mass selection, the ions fragment is accumulated inside the analyzer, one or more ions with a specific m/z ratio are selected, the others are expelled from the trap); Consecutive Reaction Monitoring (CRM) (this multistage acquisition method (from n = 3 to n = 10), allows to select the fragment ion and further fragment it, obtaining MS3 or higher, with a consequent increase in specificity). Each further fragmentation step makes the identification of the compound more precise and allows the structural characterization of the molecules of interest. Each additional fragmentation step makes compound identification more definite and enables structural characterization. The system offers molecular weight measurements with low sample volume consumption (nanoliter to microliter). APPLICATIONS: Pharmaceutical, Forensic, clinical, environmental, food, quality control, proteomics, metabolomics

• Ionization mode: ± ES
• Resolution: 0.15 FWHM; 0.3 FWHM; 0.45 FWHM; 0.7 FWHM; 3 FWHM
• Mass accuracy: Internal <1ppm RMS; external <3ppm RMS
• Polarity switching: 100msec between positive and negative
• MS scan power: MSn, n=1-10
• Manufacturer: Thermofisher Scientific™
• Instrument name: Thermo Scientific ™ LTQ XL™ Linear Ion Trap Mass Spectrometer

The liquid chromatography (LC) permits the separation of the components of a sample based on differences in their affinity or retention strength for the stationary phase and mobile phase. The Ultra High Performace Liquid Chromatography System UltiMate™ 3000 HPLC provides excellent chromatograpic performance. The system is equipped with a Quaternary Standard Pump witch supports pressures up to 62 MPa (9,000 psi/620 bar) at flow rates from 0.2 to 10 mL/min; an high presion autosampler that supports injection volumes from 1 to 100 ul; a Thermostated Column Compartment (5 to 80°C) and a Diode Array Detector with high-resolution and wavelength range of 190 to 800 nm (accurancy ±1.0 nm, noise <±8 µAU).

Applications: Bio-pharmaceutical, pharmaceutical, environmental, forensic, quality control. Manufacturer: Thermofisher Scientific™ Instrument name: Thermo Scientific ™ DIONEX UltiMate™ 3000 ™